- Open Access
Genome-wide distribution of histone H4 Lysine 16 acetylation sites and their relationship to gene expression
- Nobuo Horikoshi†1, 3Email author,
- Pankaj Kumar†4,
- Girdhar G Sharma3,
- Min Chen2,
- Clayton R Hunt1, 3,
- Kenneth Westover1,
- Shantanu Chowdhury4, 5Email author and
- Tej K Pandita1, 3Email author
© Horikoshi et al.; licensee BioMed Central Ltd. 2013
- Received: 10 February 2013
- Accepted: 4 April 2013
- Published: 12 April 2013
Histone post-translational modifications are critical determinants of chromatin structure and function, impacting multiple biological processes including DNA transcription, replication, and repair. The post-translational acetylation of histone H4 at lysine 16 (H4K16ac) was initially identified in association with dosage compensation of the Drosophila male X chromosome. However, in mammalian cells, H4K16ac is not associated with dosage compensation and the genomic distribution of H4K16ac is not precisely known. Therefore, we have mapped the genome-wide H4K16ac distribution in human cells.
We performed H4K16ac chromatin immunoprecipitation from human embryonic kidney 293 (HEK293) cells followed by hybridization to whole-genome tiling arrays and identified 25,893 DNA regions (false discovery rate <0.005) with average length of 692 nucleotides. Interestingly, although a majority of H4K16ac sites localized within genes, only a relatively small fraction (~10%) was found near promoters, in contrast to the distribution of the acetyltransferase, MOF, responsible for acetylation at K16 of H4. Using differential gene expression profiling data, 73 genes (> ±1.5-fold) were identified as potential H4K16ac-regulated genes. Seventeen transcription factor-binding sites were significantly associated with H4K16ac occupancy (p < 0.0005). In addition, a consensus 12-nucleotide guanine-rich sequence motif was identified in more than 55% of the H4K16ac peaks.
The results suggest that H4K16 acetylation has a limited effect on transcription regulation in HEK293 cells, whereas H4K16ac has been demonstrated to have critical roles in regulating transcription in mouse embryonic stem cells. Thus, H4K16ac-dependent transcription regulation is likely a cell type specific process.
- Gene expression
Covalent modifications of histone tails are thought to alter chromatin structure, thereby controlling DNA transcription, repair and replication. Acetylation of lysine 16 of histone H4 (H4K16ac) has the potential to create or secure binding platforms for transcriptional factors as well as other chromatin-modifying enzymes [1, 2]. Histone H4 lysine 16 is acetylated by males absent on the first (MOF, also called MYST1 or KAT8), a highly conserved member of the MYST histone acetyltransferase (HAT) family. MOF itself was originally discovered in Drosophila as an essential component of the X chromosome dosage compensation complex (DCC), also known as the male-specific lethal (MSL) complex. MOF increases expression of X-linked genes in male flies by 2-fold [3–6] and disruption of the MOF chromobarrel domain leads to genome-wide H4K16ac loss and compromised MSL targeting to X-linked genes . In addition to its well defined role in dosage compensation, MOF has recently been found at active promoters genome-wide in both male and female flies, where it is bound as part of the nonspecific lethal (NSL) complex [8–10]. Other members of the MYST acetyltransferase family including acute myeloid leukemia (MOZ), transcriptional silencing in Saccharomyces cerevisiae (SAS2 and YBF2/SAS3), interactions with human immunodeficiency virus Tat in humans (TIP60), are also known to have transcriptional functions as well as roles in DNA damage repair [11–19].
In mammals, MOF is essential for development. Constitutive ablation of Mof leads to peri-implantation embryonic lethality in mice [20, 21]. MOF is also essential for post-mitotic cell survival as Cre-mediated conditional MOF deletion in Purkinje cells induces chromatin blebbings and cell death, suggesting Mof has a crucial role in maintenance of chromatin structures in vivo . Histone acetylation has been suggested to have a role in both transcriptional initiation and elongation as nucleosomes present formidable barriers to the passage of Pol II during transcriptional elongation  and global acetylation in transcribed regions is required for increased basal levels of transcription in yeast [24–26]. Acetylation of nucleosomal histones in the region of transcription start sites (TSSs) may stabilize the binding of chromatin remodeling factors to promoter regions  and/or destabilize nucleosome structure [28, 29], leading to decreased nucleosome occupancy at TSSs that facilitates RNA Pol II binding . H4K16ac has been shown to impact higher order chromatin structure and create an open, highly accessible environment [31, 32] changing functional interactions between chromatin-associated proteins , and serving as a switch for altering chromatin from a repressive to a transcriptionally active state in yeast and humans .
Tip60 (Tat-interacting protein) acetylates histones H2A, H3 and H4 and plays a role in DNA repair . In Drosophila, Tip60 acetylates nucleosomal phospho-H2Av and replaces it with an unmodified H2Av . The acetylation of histone H4 by Esa1 (essential SAS2-related acetyltransferase1) is required for DNA repair in yeast  and thus a similar modification may function in mammalian cells. Depletion of human MOF (hMOF) in cells results in a corresponding H4K16ac loss [13, 19, 35–37] indicating that hMOF protein is the primary HAT responsible for histone H4 acetylation at K16. Recent studies have demonstrated that depletion of hMOF correlates with decreased DNA damage-induced activation of ATM and prevents ATM from phosphorylating downstream effectors, such as H2AX, p53 and CHK2 [13, 37]. Furthermore, hMOF physically interacts with ATM [13, 35] and hMOF binds to and acts synergistically with p53 to increase H4K16ac and target gene transcription in vitro . In yeast, the presence of H4K16ac and H2A.Z synergistically prevent the ectopic propagation of heterochromatin in subtelomeric regions .
Consistent with these genetic and biochemical results, genome-wide location analysis of HAT binding in yeast demonstrated a good correlation with transcriptional activation . Numerous combinatorial patterns of histone modifications, including those related to HATs, in human CD4+ T cells have been studied on a genome-wide scale [39, 40] showing that modification patterns change during cell differentiation [41–43]. Furthermore, recent genome-wide binding analyses demonstrated that mouse Mof plays a critical role in the Nanog-dependent transcription network in mouse embryonic stem (ES) cells . In the present studies we determine the genome-wide distribution of H4K16ac, the product of MOF acetylation, and compared it with MOF-dependent differential expression of genes in HEK293 human cancer cells. Surprisingly, the potential contribution to gene expression of H4K16ac in HEK293 cells is limited, as opposed to results from ES cell studies, suggesting that H4K16ac-dependent transcription regulation is cell type-dependent and that MOF may have other important roles in addition to transcriptional regulation through H4K14ac modification.
To determine the role of H4K16ac histone modification in regulating gene expression, we analyzed the spectrum of H4K16ac sites genome-wide for locations within or near genes. We found 5002 of the 25,893 H4K16ac sites localized in 3538 unique genes within a region encompassing 10 kb upstream and downstream of annotated TSS. Gene ontology and KEGG pathways analysis using the GeneCodis2 web-based integrative knowledgebase  for the 3538 genes revealed the highest enrichment for neuroactive ligand-receptor interactions, MAPK signaling, focal adhesion, Wnt signaling, apoptosis and cancer pathways (Figure 2C).
The H4K16ac distribution profile in HEK293 cells is similar to that observed in Drosophila and yeast [8, 52, 53]. On the Drosophila X chromosome, MOF displays a bimodal distribution, binding to the promoter and 3′ end regions of genes, whereas H4K16ac is preferentially located at the 3′ ends. Thus it seems likely that MOF distribution is not identical to that of H4K16ac and this tendency may be evolutionally conserved. MOF forms MOF-NSL complexes that are enriched at TSSs while a dosage compensation complex (DCC), also known as the MOF-MSL complex, is enriched at 3′ gene regions. Although MOF is enzymatically active in both complexes, H4K16ac is low at TSSs suggesting that the MOF-NSL complex may be either less efficient or subject to site or cell type specific inhibition. Alternatively, H4K16ac modified nucleosomes may be displaced up or down from TSS by alternative mechanisms involving transcription initiation or elongation. In support of a role for H4K16ac in transcription, a recent report demonstrated that the enhanced gene expression occurring during X chromosome dosage compensation in Drosophila is via H4K16ac-dependent enhancement of transcriptional elongation . Perhaps in some HEK293 genes transcription levels are enhanced by the distribution of H4K16ac toward 3′ ends of genes, possibly as MOF-MSL or similar complexes support transcriptional elongation.
We next analyzed H4K16ac peaks within ±10 kb of TSS for genes whose expression was altered in MOF-depleted HEK293 cells using differential gene expression profiles obtained previously from control and MOF knockdown HEK293 cells [20, 50]. Microarray profiling revealed that 1020 genes (up: 426 genes, down: 594 genes) were differentially expressed (fold change; >1.5)  which is smaller than the 4475 genes that changed more than 2-fold in expression [44, 48] in MOF conditional knockout ESCs. Fewer genes may be differentially affected by MOF-depletion in HEK293 cells due to cell type differences; ESCs are rapidly growing pluripotent cells whereas HEK293 cells are kidney cell-derived cancer cells simply undergoing constant self-renewal. Thus, transcriptional regulation by MOF may play a more significant role in ESCs than HEK293 cells, even though MOF is essential in both situations for cell viability and proliferation [20, 44]. Alternatively, the different results may be due to the fact that siRNA-mediated MOF depletion is only partial (~85%), since total depletion in 293 cells is lethal as is complete Mof gene knockout during mouse embryogenesis . Induced Mof gene knockout in mouse ESCs by Cre expression eventually results in complete loss of Mof expression [44, 48] and these cells therefore may have initiated changes related to cell death not observed during partial depletion.
In summary, the present results imply that the role of H4K16ac in transcription regulation is cell type-dependent and possibly less important in differentiated cells where other epigenetic marks, such as DNA methylation, may override histone codes . Interestingly, even though MOF dependent H4K16ac has only limited correlation with transcriptional regulation in HEK293 cells, it is still required for cell survival. Furthermore, only 13.44% of DNase I hypersensitive sites are localized within 1 kb of H4K16ac peaks in HEK293 cells (Figure 5D), suggesting that the majority of H4K16ac distributes to regions other than DNase I hypersensitive sites and loss of H4K16 acetylation, therefore, is unlikely to affect the global distribution of DNase I hypersensitive sites. Interestingly, it has been reported that the function of MOF is largely dependent on the activities of tumor suppressors . Therefore, the critical role of MOF in transcriptional regulation may be in combination with other transcriptional factors, such as FOXP3 . In other words, MOF dependent transcriptional regulation could be important only when cells are exposed to certain stimuli. Overall, our results suggest that although MOF may have a critical function in transcriptional regulation of some genes in specific cell types, additional functions other than transcriptional regulation underlie the requirement for MOF/H4K16ac for cell survival. These critical functions may be related to MOF-mediated acetylation of chromatin-associated proteins other than H4 or alterations of chromatin structure induced by such modifications that are not detectable by DNaseI sensitivity.
Identification of H4K16ac peaks in HEK293 cells
HEK293 cells were cultured as described previously [13, 20, 50]. ChIP was performed in triplicate as described  using anti-H4K16ac antibody (EMD Millipore, Billerica, MA). Recovered DNA fragments were purified and ChIP-chip was performed in Roche NimbleGen, Inc. (Madison, WI). DNA without ChIP processing was used as a reference. The differential gene expression profile was obtained from GEO (accession number: GSE20193) .
Raw ChIP-chip fluorescence intensity data obtained from NimbleGen Human ChIP-chip 2.1 M Whole-Genome Tiling −10 Array Set (probe length: 50–75 mer, median probe spacing 100 bp) were analyzed using Roche NimbleScan 2.6 software. Settings included probe intensity ratio calculation between experimental (ChIP) sample and control (non-specific IgG) sample. Enrichment is quantified by probe intensity log ratios calculated as the base 2 logarithm of the ratio between the experimental and control samples. Using the default parameters (probe length = 50 bp; P start = 90; P step = 1; # step = 76; min probes > cutoff in peak = 4; when all probes in peak > cutoff = 2 and width of sliding window = 500 bp) peaks were identified at FDR <0.005. All coordinates in this manuscript are reported in hg18 (GEO accession number: GSE44254).
Identification of an H4K16ac binding DNA motif
To identify DNA sequence motifs in the H4K16ac-bound regions detected by ChIP-chip, the sequences of all 25,893 H4K16ac-bound regions were retrieved from UCSC built hg18 and inputted into the Gibbs sampler motif finding program provided by CisGenome. The motif finder was run searching for motifs of 12 bp using 5,000 MCMC iterations and a score was produced for each motif.
Transcription factor binding site and DNase I hypersensitive site enrichment analysis
For transcription factors enrichment analysis we first generated ten control sequences by randomly picking sequences of same size from the same chromosome. Both the peak sequences and control sequences were given as input into the Match tool to scan for enriched PWMs listed in TRANSFAC professional. The total occurrence of any given TFBS on each peak sequence was considered as the observed frequency. Similarly, the occurrence of a TFBS in control set sequences, gave the randomly expected frequency. The discrepancy between observed and expected frequency was evaluated by determining the statistical variable chi-square (χ2). The presence of CTCF motif within peak sequences (FDR < 0.005) was looked for the presence of 9-mer CTCF motif (AG[GA][GT]GG[CAT][GAT][CG] and their complementary sequence). We found 13,230 unique peaks (51% of overall peaks) with at least one 9-mer CTCF motif. DNase I hypersensitive sites data for HEK293T cells (a cell line derived from HEK293) were used for our correlation analysis obtained form: http://genome.ucsc.edu/cgi-bin/hgTrackUi?hgsid=302749441&c=chr6&g=wgEncodeOpenChromDnase.
This work was supported by grants from National Institutes of Health/National Cancer Institute R01CA129537, R01CA154320, U19A1091175 and R13CA130756 to TKP, Indian Council of Medical Research (senior research fellowship to P.K.), Department of Biotechnology (Career Development Award to SC) and CSIR for research grants to SC. Thanks are due to Jerry Shay and members of the Pandita laboratory for their suggestions on the manuscript.
- Ruthenburg AJ, Li H, Milne TA, Dewell S, McGinty RK, Yuen M, Ueberheide B, Dou Y, Muir TW, Patel DJ, Allis CD: Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactions. Cell. 2011, 145: 692-706. 10.1016/j.cell.2011.03.053.PubMed CentralView ArticlePubMedGoogle Scholar
- Zippo A, Serafini R, Rocchigiani M, Pennacchini S, Krepelova A, Oliviero S: Histone crosstalk between H3S10ph and H4K16ac generates a histone code that mediates transcription elongation. Cell. 2009, 138: 1122-1136. 10.1016/j.cell.2009.07.031.View ArticlePubMedGoogle Scholar
- Conrad T, Akhtar A: Dosage compensation in Drosophila melanogaster: epigenetic fine-tuning of chromosome-wide transcription. Nat Rev Genet. 2011, 13: 123-134.View ArticleGoogle Scholar
- Gelbart ME, Kuroda MI: Drosophila dosage compensation: a complex voyage to the X chromosome. Development. 2009, 136: 1399-1410. 10.1242/dev.029645.PubMed CentralView ArticlePubMedGoogle Scholar
- Lucchesi JC: The structure-function link of compensated chromatin in Drosophila. Curr Opin Genet Dev. 2009, 19: 550-556. 10.1016/j.gde.2009.10.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Prestel M, Feller C, Straub T, Mitlohner H, Becker PB: The activation potential of MOF is constrained for dosage compensation. Mol Cell. 2010, 38: 815-826. 10.1016/j.molcel.2010.05.022.View ArticlePubMedGoogle Scholar
- Conrad T, Cavalli FM, Holz H, Hallacli E, Kind J, Ilik I, Vaquerizas JM, Luscombe NM, Akhtar A: The MOF chromobarrel domain controls genome-wide H4K16 acetylation and spreading of the MSL complex. Dev Cell. 2012, 22: 610-624. 10.1016/j.devcel.2011.12.016.View ArticlePubMedGoogle Scholar
- Kind J, Vaquerizas JM, Gebhardt P, Gentzel M, Luscombe NM, Bertone P, Akhtar A: Genome-wide analysis reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila. Cell. 2008, 133: 813-828. 10.1016/j.cell.2008.04.036.View ArticlePubMedGoogle Scholar
- Mendjan S, Taipale M, Kind J, Holz H, Gebhardt P, Schelder M, Vermeulen M, Buscaino A, Duncan K, Mueller J: Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila. Mol Cell. 2006, 21: 811-823. 10.1016/j.molcel.2006.02.007.View ArticlePubMedGoogle Scholar
- Raja SJ, Charapitsa I, Conrad T, Vaquerizas JM, Gebhardt P, Holz H, Kadlec J, Fraterman S, Luscombe NM, Akhtar A: The nonspecific lethal complex is a transcriptional regulator in Drosophila. Mol Cell. 2010, 38: 827-841. 10.1016/j.molcel.2010.05.021.View ArticlePubMedGoogle Scholar
- Bird AW, Yu DY, Pray-Grant MG, Qiu Q, Harmon KE, Megee PC, Grant PA, Smith MM, Christman MF: Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair. Nature. 2002, 419: 411-415. 10.1038/nature01035.View ArticlePubMedGoogle Scholar
- Bone JR, Lavender J, Richman R, Palmer MJ, Turner BM, Kuroda MI: Acetylated histone H4 on the male X chromosome is associated with dosage compensation in Drosophila. Genes Dev. 1994, 8: 96-104. 10.1101/gad.8.1.96.View ArticlePubMedGoogle Scholar
- Gupta A, Sharma GG, Young CS, Agarwal M, Smith ER, Paull TT, Lucchesi JC, Khanna KK, Ludwig T, Pandita TK: Involvement of human MOF in ATM function. Mol Cell Biol. 2005, 25: 5292-5305. 10.1128/MCB.25.12.5292-5305.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Hilfiker A, Hilfiker-Kleiner D, Pannuti A, Lucchesi JC: mof, a putative acetyl transferase gene related to the Tip60 and MOZ human genes and to the SAS genes of yeast, is required for dosage compensation in Drosophila. EMBO J. 1997, 16: 2054-2060. 10.1093/emboj/16.8.2054.PubMed CentralView ArticlePubMedGoogle Scholar
- Kimura A, Matsubara K, Horikoshi M: A decade of histone acetylation: marking eukaryotic chromosomes with specific codes. J Biochem. 2005, 138: 647-662. 10.1093/jb/mvi184.View ArticlePubMedGoogle Scholar
- Kusch T, Florens L, Macdonald WH, Swanson SK, Glaser RL, Yates JR, Abmayr SM, Washburn MP, Workman JL: Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions. Science. 2004, 306: 2084-2087. 10.1126/science.1103455.View ArticlePubMedGoogle Scholar
- Sterner DE, Berger SL: Acetylation of histones and transcription-related factors. Microbiol Mol Biol Rev. 2000, 64: 435-459. 10.1128/MMBR.64.2.435-459.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Suka N, Luo K, Grunstein M: Sir2p and Sas2p opposingly regulate acetylation of yeast histone H4 lysine16 and spreading of heterochromatin. Nat Genet. 2002, 32: 378-383. 10.1038/ng1017.View ArticlePubMedGoogle Scholar
- Taipale M, Rea S, Richter K, Vilar A, Lichter P, Imhof A, Akhtar A: hMOF histone acetyltransferase is required for histone H4 lysine 16 acetylation in mammalian cells. Mol Cell Biol. 2005, 25: 6798-6810. 10.1128/MCB.25.15.6798-6810.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Gupta A, Guerin-Peyrou TG, Sharma GG, Park C, Agarwal M, Ganju RK, Pandita S, Choi K, Sukumar S, Pandita RK: The mammalian ortholog of Drosophila MOF that acetylates histone H4 lysine 16 is essential for embryogenesis and oncogenesis. Mol Cell Biol. 2008, 28: 397-409. 10.1128/MCB.01045-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Thomas T, Dixon MP, Kueh AJ, Voss AK: Mof (MYST1 or KAT8) is essential for progression of embryonic development past the blastocyst stage and required for normal chromatin architecture. Mol Cell Biol. 2008, 28: 5093-5105. 10.1128/MCB.02202-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Kumar R, Hunt CR, Gupta A, Nannepaga S, Pandita RK, Shay JW, Bachoo R, Ludwig T, Burns DK, Pandita TK: Purkinje cell-specific males absent on the first (mMof) gene deletion results in an ataxia-telangiectasia-like neurological phenotype and backward walking in mice. Proc Natl Acad Sci USA. 2011, 108: 3636-3641. 10.1073/pnas.1016524108.PubMed CentralView ArticlePubMedGoogle Scholar
- Orphanides G, LeRoy G, Chang CH, Luse DS, Reinberg D: FACT, a factor that facilitates transcript elongation through nucleosomes. Cell. 1998, 92: 105-116. 10.1016/S0092-8674(00)80903-4.View ArticlePubMedGoogle Scholar
- Govind CK, Zhang F, Qiu H, Hofmeyer K, Hinnebusch AG: Gcn5 promotes acetylation, eviction, and methylation of nucleosomes in transcribed coding regions. Mol Cell. 2007, 25: 31-42. 10.1016/j.molcel.2006.11.020.View ArticlePubMedGoogle Scholar
- Vogelauer M, Wu J, Suka N, Grunstein M: Global histone acetylation and deacetylation in yeast. Nature. 2000, 408: 495-498. 10.1038/35044127.View ArticlePubMedGoogle Scholar
- Natsume-Kitatani Y, Shiga M, Mamitsuka H: Genome-wide integration on transcription factors, histone acetylation and gene expression reveals genes co-regulated by histone modification patterns. PLoS One. 2011, 6: e22281-10.1371/journal.pone.0022281.PubMed CentralView ArticlePubMedGoogle Scholar
- Hassan AH, Neely KE, Workman JL: Histone acetyltransferase complexes stabilize swi/snf binding to promoter nucleosomes. Cell. 2001, 104: 817-827. 10.1016/S0092-8674(01)00279-3.View ArticlePubMedGoogle Scholar
- Boeger H, Griesenbeck J, Strattan JS, Kornberg RD: Nucleosomes unfold completely at a transcriptionally active promoter. Mol Cell. 2003, 11: 1587-1598. 10.1016/S1097-2765(03)00231-4.View ArticlePubMedGoogle Scholar
- Reinke H, Horz W: Histones are first hyperacetylated and then lose contact with the activated PHO5 promoter. Mol Cell. 2003, 11: 1599-1607. 10.1016/S1097-2765(03)00186-2.View ArticlePubMedGoogle Scholar
- Schones DE, Cui K, Cuddapah S, Roh TY, Barski A, Wang Z, Wei G, Zhao K: Dynamic regulation of nucleosome positioning in the human genome. Cell. 2008, 132: 887-898. 10.1016/j.cell.2008.02.022.View ArticlePubMedGoogle Scholar
- Bell O, Schwaiger M, Oakeley EJ, Lienert F, Beisel C, Stadler MB, Schubeler D: Accessibility of the Drosophila genome discriminates PcG repression, H4K16 acetylation and replication timing. Nat Struct Mol Biol. 2010, 17: 894-900. 10.1038/nsmb.1825.View ArticlePubMedGoogle Scholar
- Shogren-Knaak M, Ishii H, Sun JM, Pazin MJ, Davie JR, Peterson CL: Histone H4-K16 acetylation controls chromatin structure and protein interactions. Science. 2006, 311: 844-847. 10.1126/science.1124000.View ArticlePubMedGoogle Scholar
- Shia WJ, Li B, Workman JL: SAS-mediated acetylation of histone H4 Lys 16 is required for H2A.Z incorporation at subtelomeric regions in Saccharomyces cerevisiae. Genes Dev. 2006, 20: 2507-2512. 10.1101/gad.1439206.PubMed CentralView ArticlePubMedGoogle Scholar
- Ikura T, Ogryzko VV, Grigoriev M, Groisman R, Wang J, Horikoshi M, Scully R, Qin J, Nakatani Y: Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis. Cell. 2000, 102: 463-473. 10.1016/S0092-8674(00)00051-9.View ArticlePubMedGoogle Scholar
- Dou Y, Milne TA, Tackett AJ, Smith ER, Fukuda A, Wysocka J, Allis CD, Chait BT, Hess JL, Roeder RG: Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF. Cell. 2005, 121: 873-885. 10.1016/j.cell.2005.04.031.View ArticlePubMedGoogle Scholar
- Smith ER, Cayrou C, Huang R, Lane WS, Cote J, Lucchesi JC: A human protein complex homologous to the Drosophila MSL complex is responsible for the majority of histone H4 acetylation at lysine 16. Mol Cell Biol. 2005, 25: 9175-9188. 10.1128/MCB.25.21.9175-9188.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Sykes SM, Mellert HS, Holbert MA, Li K, Marmorstein R, Lane WS, McMahon SB: Acetylation of the p53 DNA-binding domain regulates apoptosis induction. Mol Cell. 2006, 24: 841-851. 10.1016/j.molcel.2006.11.026.PubMed CentralView ArticlePubMedGoogle Scholar
- Shahbazian MD, Grunstein M: Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem. 2007, 76: 75-100. 10.1146/annurev.biochem.76.052705.162114.View ArticlePubMedGoogle Scholar
- Barski A, Cuddapah S, Cui K, Roh TY, Schones DE, Wang Z, Wei G, Chepelev I, Zhao K: High-resolution profiling of histone methylations in the human genome. Cell. 2007, 129: 823-837. 10.1016/j.cell.2007.05.009.View ArticlePubMedGoogle Scholar
- Wang Z, Zang C, Rosenfeld JA, Schones DE, Barski A, Cuddapah S, Cui K, Roh TY, Peng W, Zhang MQ, Zhao K: Combinatorial patterns of histone acetylations and methylations in the human genome. Nat Genet. 2008, 40: 897-903. 10.1038/ng.154.PubMed CentralView ArticlePubMedGoogle Scholar
- Cui K, Zang C, Roh TY, Schones DE, Childs RW, Peng W, Zhao K: Chromatin signatures in multipotent human hematopoietic stem cells indicate the fate of bivalent genes during differentiation. Cell Stem Cell. 2009, 4: 80-93. 10.1016/j.stem.2008.11.011.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang Z, Schones DE, Zhao K: Characterization of human epigenomes. Curr Opin Genet Dev. 2009, 19: 127-134. 10.1016/j.gde.2009.02.001.PubMed CentralView ArticlePubMedGoogle Scholar
- Wei G, Wei L, Zhu J, Zang C, Hu-Li J, Yao Z, Cui K, Kanno Y, Roh TY, Watford WT: Global mapping of H3K4me3 and H3K27me3 reveals specificity and plasticity in lineage fate determination of differentiating CD4+ T cells. Immunity. 2009, 30: 155-167. 10.1016/j.immuni.2008.12.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Li X, Li L, Pandey R, Byun JS, Gardner K, Qin Z, Dou Y: The histone acetyltransferase MOF is a key regulator of the embryonic stem cell core transcriptional network. Cell Stem Cell. 2012, 11: 163-178. 10.1016/j.stem.2012.04.023.PubMed CentralView ArticlePubMedGoogle Scholar
- Millar CB, Kurdistani SK, Grunstein M: Acetylation of yeast histone H4 lysine 16: a switch for protein interactions in heterochromatin and euchromatin. Cold Spring Harb Symp Quant Biol. 2004, 69: 193-200. 10.1101/sqb.2004.69.193.View ArticlePubMedGoogle Scholar
- Vaquero A, Scher MB, Lee DH, Sutton A, Cheng HL, Alt FW, Serrano L, Sternglanz R, Reinberg D: SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis. Genes Dev. 2006, 20: 1256-1261. 10.1101/gad.1412706.PubMed CentralView ArticlePubMedGoogle Scholar
- Carmona-Saez P, Chagoyen M, Tirado F, Carazo JM, Pascual-Montano A: GENECODIS: a web-based tool for finding significant concurrent annotations in gene lists. Genome Biol. 2007, 8: R3-10.1186/gb-2007-8-1-r3.PubMed CentralView ArticlePubMedGoogle Scholar
- Megee PC, Morgan BA, Mittman BA, Smith MM: Genetic analysis of histone H4: essential role of lysines subject to reversible acetylation. Science. 1990, 247: 841-845. 10.1126/science.2106160.View ArticlePubMedGoogle Scholar
- Lawrence CE, Altschul SF, Boguski MS, Liu JS, Neuwald AF, Wootton JC: Detecting subtle sequence signals: a Gibbs sampling strategy for multiple alignment. Science. 1993, 262: 208-214. 10.1126/science.8211139.View ArticlePubMedGoogle Scholar
- Sharma GG, So S, Gupta A, Kumar R, Cayrou C, Avvakumov N, Bhadra U, Pandita RK, Porteus MH, Chen DJ: MOF and histone H4 acetylation at lysine 16 are critical for DNA damage response and double-strand break repair. Mol Cell Biol. 2010, 30: 3582-3595. 10.1128/MCB.01476-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang Z, Zang C, Cui K, Schones DE, Barski A, Peng W, Zhao K: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes. Cell. 2009, 138: 1019-1031. 10.1016/j.cell.2009.06.049.PubMed CentralView ArticlePubMedGoogle Scholar
- Heise F, Chung HR, Weber JM, Xu Z, Klein-Hitpass L, Steinmetz LM, Vingron M, Ehrenhofer-Murray AE: Genome-wide H4 K16 acetylation by SAS-I is deposited independently of transcription and histone exchange. Nucleic Acids Res. 2012, 40: 65-74. 10.1093/nar/gkr649.PubMed CentralView ArticlePubMedGoogle Scholar
- Larschan E, Bishop EP, Kharchenko PV, Core LJ, Lis JT, Park PJ, Kuroda MI: X chromosome dosage compensation via enhanced transcriptional elongation in Drosophila. Nature. 2011, 471: 115-118. 10.1038/nature09757.PubMed CentralView ArticlePubMedGoogle Scholar
- Feller C, Prestel M, Hartmann H, Straub T, Soding J, Becker PB: The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset. Nucleic Acids Res. 2012, 40: 1509-1522. 10.1093/nar/gkr869.PubMed CentralView ArticlePubMedGoogle Scholar
- Bhadra MP, Horikoshi N, Pushpavallipvalli SN, Sarkar A, Bag I, Krishnan A, Lucchesi JC, Kumar R, Yang Q, Pandita RK: The role of MOF in the ionizing radiation response is conserved in Drosophila melanogaster. Chromosoma. 2012, 121: 79-90. 10.1007/s00412-011-0344-7.PubMed CentralView ArticlePubMedGoogle Scholar
- Jones PA: Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nat Rev Genet. 2012, 13: 484-492. 10.1038/nrg3230.View ArticlePubMedGoogle Scholar
- Katoh H, Qin ZS, Liu R, Wang L, Li W, Li X, Wu L, Du Z, Lyons R, Liu CG: FOXP3 orchestrates H4K16 acetylation and H3K4 trimethylation for activation of multiple genes by recruiting MOF and causing displacement of PLU-1. Mol Cell. 2011, 44: 770-784. 10.1016/j.molcel.2011.10.012.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.