H4K16ac distribution in genes differentially regulated by MOF status. (A) H4K16ac peak distribution in differentially expressed genes. H4K14ac peaks are mapped within ±10 kb TSS with pink bars for each gene and the total H4K16ac distribution is shown in lower panel. Genes are aligned according to relative changes in expression of the transcript as determined by microarrays on targeted depletion of H4K16ac in HEK293 cells. (B) H4K16ac distribution in down-regulated genes (less than −1.5-fold). (C) H4K16ac distribution in up-regulated genes (more than 1.5-fold). (D) DNase I hypersensitive sites are poorly correlated with H4K16ac peaks in HEK293 cells. Total H4K16ac peaks were analyzed for DNase I hypersensitive sites. As shown in table, only 13.44% of H4K16ac peaks also contain DNase I hypersensitive sites within the region ±1 kb from the peak.