Figure 1

Kinetic effects of products of oxidative DNA damage in SMRT sequencing. Normalized IPD ratios (top panel) and PW ratios (bottom panel) between the modified and unmodified DNA templates are shown for (a) 8oxoG and (b) 8oxoA, with the DNA template sequence as the x axis. The two template positions carrying the base modifications are highlighted with red bars and underlined sequence labels. The direction of DNA polymerization (left to right, thus the DNA template sequence is 3' to 5') as well as the coordinate space relative to the modified sites as referred to in the text is indicated in (a) and omitted for clarity in the rest of the figures.