Figure 3

Telo-FISH result following DNA-PKcs inhibition in human Artemis defective primary cell lines. Two Artemis defective (CJ179, F01/240) and normal (GM08399) human primary cell lines were subjected to 200μM of DNA-PKcs inhibitor, IC86621, for twenty-four hour period. Total levels of telomeric fusions, including chromosome type, chromatid type, sister chromatic unions and chromosome ring fusions were scored in three independent experiments. Panel (A) is DMSO treated and panel (B) is DNA-PKcs inhibited. Levels of DNA-DSB chromatid fragments were also scored as breaks/fragments. Error bars indicate standard error of mean (SEM).