FANCD2 immunoblotting of fibroblast extracts from FA-D2 patients and control cell lines. Exposure of the 1823, 1866 (2 cell lines from the same patient) and 1879 patient cell lines to 50nM MMC for 24 h and subsequent analysis by Western blot (loading control Vinculin, L: lane for the Ladder). A Assignment to group FA-D2 of 18663 and 18665 patient cell lines on the basis of the absence FANCD2 on Western blot. B Assignment to group FA-D2 of 1823 and 1879 patient cell lines on the basis of the absence FANCD2 band on Western blot. C Over-exposure of immunoblots reveals that 1823, 18663, 18665 and 1879 patient cell lines show faint but visible FANCD2 bands in response to MMC.