B-NHEJ kinetics in TSA-treated exponentially growing Lig4-/-MEFs. (A) Western blot showing H3K9Ac acetylation at the time of IR exposure 4 h after TSA administration, as well as at different times after drug removal. DR - cells used for the dose response curve; correspond to acetylation measured after 4 h TSA treatment; Ctrl – cells treated with DMSO. (B) Cell cycle distribution of control and TSA-treated cells employed in DSB repair experiments. Cells were analyzed immediately before exposure to IR. (C) Induction of DSBs in cells treated as indicated. Other details are as in Figure 1B. (D) Kinetics of rejoining of IR induced DSBs in control and TSA-treated cells incubated for repair in the presence (+) or absence (-) of TSA. The results shown represent the mean and standard error calculated from 6 determinations in 2 independent experiments. Other details are as in Figure 1C.