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Table 6 Primer sequences and conditions employed in RT-PCR

From: Differential regulation of intracellular factors mediating cell cycle, DNA repair and inflammation following exposure to silver nanoparticles in human cells

Gene Primer sequence TA (°C)
p53 5'GGC CCA CTT CAC CGT ACT AA3'
5'GTG GTT TCA AGG CCA GAT GT3'
60
Mitogen activated protein
kinase (MAPK1)
5'CCA CCC ATA TCT GGA GCA GT3'
5'CAG TCC TCT GAG CCC TTG TC 3'
60
Interleukin-8 (IL-8) 5'ATG ACT TCC AAG CTG GCC GTG GCT 3'
5'TCT CAG CCC TCT TCA AAA ACT TCT C 3'
61
Nuclear factor beta (NFκB) 5' AGT TGA GGG GAC TTT CCC AGG 3'
5' GCC TGG GAA AGT CCC CTC AAC T 3'
61
18s Ribosomal subunit 5' GTA ACC CGT TGA ACC CCA TT-3'
5' CCA TCC AAT CGG TAG TAG CG 3'
60