Table 6 Primer sequences and conditions employed in RT-PCR
Gene | Primer sequence | TA (°C) |
|---|---|---|
p53 | 5'GGC CCA CTT CAC CGT ACT AA3' 5'GTG GTT TCA AGG CCA GAT GT3' | 60 |
Mitogen activated protein kinase (MAPK1) | 5'CCA CCC ATA TCT GGA GCA GT3' 5'CAG TCC TCT GAG CCC TTG TC 3' | 60 |
Interleukin-8 (IL-8) | 5'ATG ACT TCC AAG CTG GCC GTG GCT 3' 5'TCT CAG CCC TCT TCA AAA ACT TCT C 3' | 61 |
Nuclear factor beta (NFκB) | 5' AGT TGA GGG GAC TTT CCC AGG 3' 5' GCC TGG GAA AGT CCC CTC AAC T 3' | 61 |
18s Ribosomal subunit | 5' GTA ACC CGT TGA ACC CCA TT-3' 5' CCA TCC AAT CGG TAG TAG CG 3' | 60 |