Figure 9
Average number of 53BP1 foci/nucleus observed at different times after exposure to 2 Gy γ-rays categorised into three size integers; < 0.5 μm (white), 0.5-1 μm (grey), > 1 μm (dark grey) and out-of-focus (black) scored by manual 2D and 3D analysis (A&C) and AutoRIF 2D and 3D analysis (B&D) of the same images. Averages are from at least two independent experiments.