Figure 6

Effects of RIF filtering steps. Pre-laplacian, laplacian and post-laplacian filtering effects on an image of a single nucleus with 53BP1 foci. The optimal filtering combination for this nucleus would be 3 pre-filtering steps, medium sensitivity laplacian filter and 3 post-laplacian filtering steps. ~10% of nuclei are batch tested (covering test and sham slides) to identify the optimal combination of filters to be used for each experiment.