Workflow for image processing and analysis. Workflow A-F shows the process for analysing images in each experiment. Panel 1 is an example of a greyscale raw image with nucleus outlined and 53BP1 foci visible. Panel 2 shows a binary image with minimal thresholding and an arrow highlighting salt & pepper noise while panel 3 shows the effects of increasing filtering resulting in altered morphology of foci. Panel 4 is a compromise that generates a binary image that more closely resembles the raw image.