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Figure 5 | Genome Integrity

Figure 5

From: Development and validation of 'AutoRIF': software for the automated analysis of radiation-induced foci

Figure 5

Workflow for image processing and analysis. Workflow A-F shows the process for analysing images in each experiment. Panel 1 is an example of a greyscale raw image with nucleus outlined and 53BP1 foci visible. Panel 2 shows a binary image with minimal thresholding and an arrow highlighting salt & pepper noise while panel 3 shows the effects of increasing filtering resulting in altered morphology of foci. Panel 4 is a compromise that generates a binary image that more closely resembles the raw image.

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