Figure 3

BRCA2 knock-down using siRNA oligonucleotides in U2OS and HeLa cells. A. Relative quantities (RQ) of BRCA2 mRNA assessed by quantitative real time PCR after various times following transfection with siRNA oligonucleotides specific for BRCA2. Error bars represent SD. B. Western blot analysis of BRCA2 expression following transfection with siRNA oligonucleotides. C. Frequencies of T-SCEs in U2OS and HeLa cells after BRCA2 depletion. T-SCE frequencies were measured 72 hrs after transfection with siRNA oligonucleotides. A minimum of 50 cells have been analyzed in two independent experiments. Error bars represent SEM.