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Table 3 Effect of lig4Δ, exo1Δ, rad1Δ, pol32 Δ and yen1 Δ mutations on the accumulation of GCRs in checkpoint-proficient and checkpoint-deficient sgs1 Δ mutants

From: Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

Relevant genotypea GCR rateb 95% CIc
wildtype 1.1 < 1-6.2
exo1 24 7-79
sgs1 220 144-276
sgs1 mec3 1297 1120-2030
exo1 sgs1 43800 30400-186000
exo1 mec3 30 12-39
exo1 mec3 sgs1 1168498 549530-3251000
sgs1 mec3 exo1 lig4 895988 701149-1236740
lig4 16 ND
sgs1 lig4 80 35-254
sgs1 mec3 lig4 1335 948-2140
yen1 < 5 < 4-6
sgs1 yen1 81 57-265
sgs1 mec3 yen1 1089 254-2540
pol32 20 15-26
sgs1 pol32 25 < 24-105
sgs1 mec3 pol32 2317 1800-3110
rad1 10 < 9-23
sgs1 rad1 63 25-356
sgs1 mec3 rad1 1173 1020-1540
  1. a Strains with multiple gene deletions were constructed by sporulation of the appropriate heterozygous diploids. GCR rates with 95% confidence intervals (CIs) for wildtype [81], sgs1 [82], sgs1 mec3 [60] and lig4 [1] were reported previously and are included for comparison. Spores with both sgs1Δ and pol32Δ mutations grew very slowly and exhibited a low viable cell count on YPD in the GCR assay.
  2. b The rate of accumulating gross-chromosomal rearrangements (GCRs) is calculated by selecting for cells resistant to canavanine (Canr) and 5-fluoro-orotic acid (5-FOAr) and is expressed as Canr 5-FOAr × 10-10 [77].
  3. c 95% confidence intervals (CI) for median GCR rates were calculated according to Nair [80].