Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

Table 3 Effect of lig4Δ, exo1Δ, rad1Δ, pol32 Δ and yen1 Δ mutations on the accumulation of GCRs in checkpoint-proficient and checkpoint-deficient sgs1 Δ mutants

^a Strains with multiple gene deletions were constructed by sporulation of the appropriate heterozygous diploids. GCR rates with 95% confidence intervals (CIs) for wildtype [81], sgs1 [82], sgs1 mec3 [60] and lig4 [1] were reported previously and are included for comparison. Spores with both sgs1Δ and pol32Δ mutations grew very slowly and exhibited a low viable cell count on YPD in the GCR assay.
^b The rate of accumulating gross-chromosomal rearrangements (GCRs) is calculated by selecting for cells resistant to canavanine (Can^r) and 5-fluoro-orotic acid (5-FOA^r) and is expressed as Can^r 5-FOA^r × 10^-10 [77].
^c 95% confidence intervals (CI) for median GCR rates were calculated according to Nair [80].