Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

Table 2 Effect of homologous recombination mutations on the ability of the sgs1 mec3 mutant to accumulate GCRs and form rearrangements between the CAN1, LYP 1 and ALP1 genes.

^a Median rate of cells resistant to canavanine and 5-FOA (Can^r 5-FOA^r × 10^-10). 95% confidence intervals (CIs) for median GCR rates were calculated according to Nair [80], where non-overlapping confidence intervals indicate statistically significant differences between median GCR rates. GCR rates for wildtype [81], sgs1 [82], mec3, sgs1 mec3 [60], rad51, sgs1 mec3 rad51 and sgs1 mec3 rad52 mutants [10] were reported previously and are included for comparison.
^b Rate of accumulating translocations between CAN1, LYP1 and/or ALP1 (Can^r 5-FOA^r × 10^-10). GCR clones from sgs1, mec3, sgs1 mec3, sgs1 mec3 rad51, sgs1 mec3 rad52 were previously screened for CAN1/LYP1/ALP1 translocations [10, 60]. ND, not determined.
^c To determine 95% CIs for sgs1 mec3 rad51 and sgs1 mec3 rad52 mutants, GCR rates were re-measured for the current study. The GCR rate for the sgs1 mec3 rad51 mutant was 1933 × 10^-10 (95% CIs: 601-2240 × 10^-10) and the GCR rate for the sgs1 mec3 rad52 mutant was 2220 × 10^-10 (951-3470 × 10^-10). The previously reported rates fall within the 95% CIs determined in the current study.