Skip to main content

Table 2 Effect of homologous recombination mutations on the ability of the sgs1 mec3 mutant to accumulate GCRs and form rearrangements between the CAN1, LYP 1 and ALP1 genes.

From: Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

  All GCR
typesa
CAN1/LYP1/ALP1
translocationsb
Relevant genotype Rate 95% CI Rate Frequency
wildtype 1.1 < 1-6.2 ND ND
rad51 a < 8 < 7-15 ND ND
rad52 138 16-267 ND ND
rad59 24 13-50 ND ND
sgs1 a 220 144-276 < 7.3 0/30
mec3 a 46 18-75 < 1.5 0/30
sgs1 rad59 126 107-300 ND ND
sgs1 rad59 rad51 118 49-154 ND ND
sgs1 mec3 a 1297 1120-2030 173 20/150
sgs1 mec3 rad51 a 1491 NDc 198 4/30
sgs1 mec3 rad52 a 3168 NDc < 23 0/136
sgs1 mec3 rad59 2476 1595-3187 16 1/158
sgs1 mec3 rad59 rad51 1124 734-1460 7 1/168
  1. a Median rate of cells resistant to canavanine and 5-FOA (Canr 5-FOAr × 10-10). 95% confidence intervals (CIs) for median GCR rates were calculated according to Nair [80], where non-overlapping confidence intervals indicate statistically significant differences between median GCR rates. GCR rates for wildtype [81], sgs1 [82], mec3, sgs1 mec3 [60], rad51, sgs1 mec3 rad51 and sgs1 mec3 rad52 mutants [10] were reported previously and are included for comparison.
  2. b Rate of accumulating translocations between CAN1, LYP1 and/or ALP1 (Canr 5-FOAr × 10-10). GCR clones from sgs1, mec3, sgs1 mec3, sgs1 mec3 rad51, sgs1 mec3 rad52 were previously screened for CAN1/LYP1/ALP1 translocations [10, 60]. ND, not determined.
  3. c To determine 95% CIs for sgs1 mec3 rad51 and sgs1 mec3 rad52 mutants, GCR rates were re-measured for the current study. The GCR rate for the sgs1 mec3 rad51 mutant was 1933 × 10-10 (95% CIs: 601-2240 × 10-10) and the GCR rate for the sgs1 mec3 rad52 mutant was 2220 × 10-10 (951-3470 × 10-10). The previously reported rates fall within the 95% CIs determined in the current study.