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Table 1 Functional interaction between Sgs1 and components of the DNA-damage checkpoint in the suppression of GCRs and translocations between CAN1, LYP 1 and ALP1 genes.

From: Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

  All GCR typesa CAN1/LYP1/ALP1translocationsb Frequency of CAN1/LYP1/ALP1
translocation typesc
Relevant Genotyped Rate 95% CI Rate Frequency CAN1-ALP1 CAN1-LYP1 CAN1-LYP1-ALP1
wildtype 1.1 < 1 - 6.2 ND ND ND ND ND
sgs1 220 144-276 < 7.3 0/30 0/30 0/30 0/30
rad17 57 26-74 ND ND ND ND ND
mec3 46 18-75 < 1.5 0/30 0/30 0/30 0/30
mec3 rad17 49 32-64 ND ND ND ND ND
sgs1 rad17 2515 903-4160 < 101 0/25 0/25 0/25 0/25
sgs1 mec3 1297 1120-2030 173 20/150 7/150 3/150 7/150
sgs1 mec3 rad17 1690 1247-2230 75 2/45 1/45 1/45 0/45
tel1 2 ND ND ND ND ND ND
tel1 mec3 453 340-638 15 1/30 1/30 0/30 0/30
tel1 rad17 129 73-246 < 8.6 0/15 0/15 0/15 0/15
sgs1 tel1 227 46-418 NDb ND ND ND ND
sgs1 tel1 rad17 27600 22430-39653 4600 6/36 1/36 1/36 4/36
sgs1 tel1 mec3 57370 47157-76301 2674 11/236 0/236 6/236 4/236
sgs1 tel1 mec3 rad17 31960 23400-51800 ND ND ND ND ND
mec1 471 209-859 ND ND ND ND ND
sgs1 mec1 1930 960-2452 < 10 0/190 0/190 0/190 0/190
sgs1 mec1 mec3 9628 5870-12100 < 22 0/431 0/431 0/431 0/431
chk1 42 25-132 ND ND ND ND ND
sgs1 chk1 446 337-528 < 15 0/30 0/30 0/30 0/30
sgs1 chk1 mec3 1099 725-1613 147 4/30 1/30 0/30 3/30
dun1 252 86-472 ND ND ND ND ND
sgs1 dun1 1145 698-1910 < 23 0/50 0/50 0/50 0/50
sgs1 dun1 mec3 2800 2270-3570 < 21 0/135 0/135 0/135 0/135
  1. a GCR rate (Canr 5-FOAr × 10-10). 95% confidence intervals (CIs) for median GCR rates were calculated according to Nair [80], where non-overlapping confidence intervals indicate statistically significant differences between median GCR rates. GCR rates of wildtype [81], sgs1 [82], mec3, sgs1 mec3 [60], tel1 [3] were reported previously.
  2. b Rate of accumulating translocations between CAN1, LYP1 and/or ALP1 genes (x 10-10). GCR clones from sgs1, mec3, sgs1 mec3, sgs1 tel1 and sgs1 mec1 were previously screened for CAN1/LYP1/ALP1 translocations [10, 60].
  3. c Types of CAN1/LYP1/ALP1 translocations were determined by sequencing. Of the 20 CAN1/LYP1/ALP1 translocations identified among 150 GCR clones from the sgs1 mec3 mutant, 17 were identified as being either C/A, C/L/A or C/L translocations and 3 clones had a mixture of multiple translocations [60].
  4. d All mutants with a mec1 deletion also contain a sml1 deletion.