Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability

Table 1 Functional interaction between Sgs1 and components of the DNA-damage checkpoint in the suppression of GCRs and translocations between CAN1, LYP 1 and ALP1 genes.

	All GCR types^a		CAN1/LYP1/ALP1translocations^b		Frequency of CAN1/LYP1/ALP1 translocation types^c
Relevant Genotype^d	Rate	95% CI	Rate	Frequency	CAN1-ALP1	CAN1-LYP1	CAN1-LYP1-ALP1
wildtype	1.1	< 1 - 6.2	ND	ND	ND	ND	ND
sgs1	220	144-276	< 7.3	0/30	0/30	0/30	0/30
rad17	57	26-74	ND	ND	ND	ND	ND
mec3	46	18-75	< 1.5	0/30	0/30	0/30	0/30
mec3 rad17	49	32-64	ND	ND	ND	ND	ND
sgs1 rad17	2515	903-4160	< 101	0/25	0/25	0/25	0/25
sgs1 mec3	1297	1120-2030	173	20/150	7/150	3/150	7/150
sgs1 mec3 rad17	1690	1247-2230	75	2/45	1/45	1/45	0/45
tel1	2	ND	ND	ND	ND	ND	ND
tel1 mec3	453	340-638	15	1/30	1/30	0/30	0/30
tel1 rad17	129	73-246	< 8.6	0/15	0/15	0/15	0/15
sgs1 tel1	227	46-418	ND^b	ND	ND	ND	ND
sgs1 tel1 rad17	27600	22430-39653	4600	6/36	1/36	1/36	4/36
sgs1 tel1 mec3	57370	47157-76301	2674	11/236	0/236	6/236	4/236
sgs1 tel1 mec3 rad17	31960	23400-51800	ND	ND	ND	ND	ND
mec1	471	209-859	ND	ND	ND	ND	ND
sgs1 mec1	1930	960-2452	< 10	0/190	0/190	0/190	0/190
sgs1 mec1 mec3	9628	5870-12100	< 22	0/431	0/431	0/431	0/431
chk1	42	25-132	ND	ND	ND	ND	ND
sgs1 chk1	446	337-528	< 15	0/30	0/30	0/30	0/30
sgs1 chk1 mec3	1099	725-1613	147	4/30	1/30	0/30	3/30
dun1	252	86-472	ND	ND	ND	ND	ND
sgs1 dun1	1145	698-1910	< 23	0/50	0/50	0/50	0/50
sgs1 dun1 mec3	2800	2270-3570	< 21	0/135	0/135	0/135	0/135

^a GCR rate (Can^r 5-FOA^r × 10^-10). 95% confidence intervals (CIs) for median GCR rates were calculated according to Nair [80], where non-overlapping confidence intervals indicate statistically significant differences between median GCR rates. GCR rates of wildtype [81], sgs1 [82], mec3, sgs1 mec3 [60], tel1 [3] were reported previously.
^b Rate of accumulating translocations between CAN1, LYP1 and/or ALP1 genes (x 10^-10). GCR clones from sgs1, mec3, sgs1 mec3, sgs1 tel1 and sgs1 mec1 were previously screened for CAN1/LYP1/ALP1 translocations [10, 60].
^c Types of CAN1/LYP1/ALP1 translocations were determined by sequencing. Of the 20 CAN1/LYP1/ALP1 translocations identified among 150 GCR clones from the sgs1 mec3 mutant, 17 were identified as being either C/A, C/L/A or C/L translocations and 3 clones had a mixture of multiple translocations [60].
^d All mutants with a mec1 deletion also contain a sml1 deletion.