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Table 2 Gene amplification analysis in MTX-resistant clones

From: Gene amplification in human cells knocked down for RAD54

    N° (%) MTX-resistant clones with
Knocked-down
gene
N° clones
analyzed
N° amplified
clones (%)
HSR DMs HSR DMs i(5q) DMs i(5q) HSR DMs i(5q)
None (HeLa wt) 11 4 (36%) 0 4 (100%) 0 0 0 0
DNA-PKcs 24 16 (67%) 0 14 (87.5%) 1 (6.2%) 0 0 1 (6.2%)
RAD54 24 16 (67%) 1 (6.2%) 6 (37.5%) 3 (18.7%) 4 (25%) 2 (12.5%) 0
RAD54 and DNA-PKcs 11 7 (64%) 2 (28.6%) 1 (14.3%) 0 3 (42.8%) 1 (14.3%) 0
  1. The clones analyzed derived from cell lines with different repair functions impaired. The organization of the DHFR gene was analyzed on mitotic chromosomes by FISH with a specific DHFR probe. HSR: homologous staining regions, DMs: double minutes, i(5q): isochromosome.