Table 2 Gene amplification analysis in MTX-resistant clones
From: Gene amplification in human cells knocked down for RAD54
|  |  |  | N° (%) MTX-resistant clones with | |||||
|---|---|---|---|---|---|---|---|---|
Knocked-down gene | N° clones analyzed | N° amplified clones (%) | HSR | DMs | HSR DMs | i(5q) | DMs i(5q) | HSR DMs i(5q) |
None (HeLa wt) | 11 | 4 (36%) | 0 | 4 (100%) | 0 | 0 | 0 | 0 |
DNA-PKcs | 24 | 16 (67%) | 0 | 14 (87.5%) | 1 (6.2%) | 0 | 0 | 1 (6.2%) |
RAD54 | 24 | 16 (67%) | 1 (6.2%) | 6 (37.5%) | 3 (18.7%) | 4 (25%) | 2 (12.5%) | 0 |
RAD54 and DNA-PKcs | 11 | 7 (64%) | 2 (28.6%) | 1 (14.3%) | 0 | 3 (42.8%) | 1 (14.3%) | 0 |