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Table 1 Relative gene amplification ability

From: Gene amplification in human cells knocked down for RAD54

  Ratio versusHeLa parental line
Cell lines 45 nM MTX 55 nM MTX
  1 2 3 4 Mean 1 2 3 4 Mean
Hela 1 1 1 1 1 1 1 n.a 1 1
pSSP1 2.8 0.6 1.7 1.1 1.5 2.0 0.7 n.a. 0.9 1.2
CRPK 1.1 6.4 3.0 17.3 n.a. 8.9 3.5 3.1 n.a. n.a. 3.3
CRPK 1.4 11.4 2.9 18.3 2.1 8.7 4.5 5.1 n.a. 4.5 4.7
shRAD54 3.1b 2.2 2.8 2.0 n.a. 2.3 2.0 2.1 n.a. n.a 2.0
shRAD54 3.1c 5.0 1.4 3.0 3.4 3.2 3.0 1.1 n.a. 5.3 3.1
shRAD54/CRPK 2b n.a. 0.3 1.1 0.8 0.7 n.a 0.3 n.a. 1.3 0.8
shRAD54/CRPK 5a 0.4 0.3 0.6 1.4 0.7 0.5 0.3 n.a. 1.2 0.4
  1. Cell lines with different repair function impaired were analyzed together with their respective controls. The values represent the ratio between the frequency of MTX-resistant clones in each cell line versus the HeLa parental cell line in four different experiments and at two different MTX concentrations. n.a.: not-analyzed.