Figure 4
From: Gene amplification in human cells knocked down for RAD54
Cytogenetic structure of DHFR gene amplicons. Upper section of the panel: metaphase spreads hybridized with the BAC clone containing the DHFR gene (red signal) showing (a) a DHFR gene amplicon organized as a ladder, (b) DMs and (c) i(5q). Lower section of the panel: examples of different ladder-like structures (d-g) and i(5q) (h-i) found in MTX-resistant clones analyzed using double-colour FISH with the BAC containing the DHFR gene (green signal) and the BAC RP11-297G19 (red signal).