Figure 2
From: Gene amplification in human cells knocked down for RAD54
Sensitivity to γ-irradiation. The results of three different experiments (A, B and C) are reported in which the following cell lines were analyzed: single RAD54 inhibited (shRAD54 3.1c and shRAD54 3.1b), single DNA-PKcs inhibited (CRPK1-4) and double RAD54/DNA-PKcs (shRAD54/CRPK 2b and shRAD54/CRPK 5a) inhibited cell lines. The HeLa parental cell line and cell line stably transfected with the scrambled plasmid (shRAD54 scrambled) were used as control. Survival to irradiation for all cell lines was measured in duplicate samples using a clonogenic assay.