Binding of FANCD2 and TelDNA. (A) Experimental scheme showing specific binding of recombinant FANCD2 protein from cell extracts with TelDNA oligoduplexes. Duplex oligonucleotides containing TelDNA sequence were [32P]-end labeled and incubated with 100 μg of PD20 or PD20cor cell extracts. Complexes were separated and either subjected to autoradiography (B) or transferred to nitrocellulose for Western blot analysis with anti-FANCD2 antibody (C). The position of specific complexes formed with 32P-TelDNA and proteins from FANCD2-/- (PD20) or FANCD2-/- corrected (PD20cor) cell extracts separated on native gel matches the FANCD2 signals from the corresponding immunoblot. These specific complexes are not seen in PD20 samples.