Figure 1
From: Fanconi anemia protein FANCD2 inhibits TRF1 polyADP-ribosylation through tankyrase1-dependent manner
FANCD2 binds to telomere DNA sequence. (A) FANCD2-dependent DNase I protection assay show degradation of 32P-labeled 600 bp DNA containing either two 48 bp inserts of tandem telomeric sequences (lanes 1-9) or nonTelDNA of the same length (lanes 10-12) upon treatment with increased concentrations of DNaseI (lanes 2-11), as described in Experimental procedures. Myelin binding protein (MBP) was added as a non-FANCD2 negative control; (B) Mobility shift assay show that incubation of [32P]-labeled 40 bp telomere DNA with recombinant full length FANCD2 protein resulted in protein-DNA complex formation (lane1) identified on native gels. This complex was competed by increasing amounts of cold DNA, indicating the specificity of the telomere binding (lanes 2-4). Please note, that DNAse concentrations are comparable in lanes 3,7,10 and 4,8,11.