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Figure 2 | Genome Integrity

Figure 2

From: ATM mediated phosphorylation of CHD4 contributes to genome maintenance

Figure 2

CHD4 is phosphorylated in an ATM-dependent manner at Ser1349 following DNA damage. (A) C3ABR cells were either mock irradiated or treated with 10Gy IR, 1hr after treatment cell lysates were prepared, immunoprecipitated for ATM and then an ATM kinase assay was performed against GST-CHD4 fusion protein fragments as indicated. (B) ATM proficient (C3ABR) and ATM deficient (L3) cells were irradiated with 10Gy IR, 1hr after IR cell lysates were prepared and immunoprecipitated for ATM and an ATM kinase assay was then performed against 1 μg of purified GST-CHD4 fusion protein fragments as indicated. (C) Mock (siControl) depleted or CHD4 depleted (siCHD4) U2OS cells were either untreated or treated with 6Gy IR, 1hr after IR cell lysates were prepared and subjected to western blot analysis for CHD4S1349 and CHD4 as indicated. (D) Hela cells and CaCO2 cells were either untreated or treated with 6Gy IR, 1hr after IR cell lysates were prepared and subjected to western blot analysis for CHD4S1349 and CHD4 as indicated. (E) ATM proficient (C3ABR) cells, ATM deficient (L3) cells and ATR deficient (Seckle) cells were either untreated or treated with 6Gy IR, cell lysates were then prepared at 30 min and 1hr after IR or from unirradiated cells. The lysates were then subjected to western blot analysis as indicated.

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