Cell cycle analysis in UHRF1-depleted cells following γ-irradiation. UHRF1 or control shRNA-expressing cells were pulsed with BrdU and then treated with 1 Gy (A) or 5 Gy (B) of γ-irradiation. Cells were harvested at the indicated times and stained with PI and for BrdU. For each time point, representative DNA content histograms (PI stain) are shown on the left with corresponding BrdU-PI bivariate plots on the right. Numerical values indicate percentage of G1, S and G2/M cells for each time point. The percentage change of cells in either G1 phase (C), S phase (D) or G2/M phases (E) compared to non-irradiated cells are plotted as mean ± S.D. of three independent experiments (shown for 1 Gy).