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Table 2 Analysis of metaphase spreads of lymphoblastoids following H2O2 treatment

From: Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

    Fusions Breaks   
Cell Type H2O2(μM) Chromosomes per metaphase T/QLS SCF D/T R EAF Total Fusions CB F UT Total Breaks Total Aberrations Aberrant cells (%)
  0 45.90 ± 0.68
(44-48) 		0 0 0 0 0 0 0 1 6 7 7 7
  20 46.04 ± 0.49
(44-48) 		0 1 0 0 1 2 0 1 10 11 13 10
Normal-L 40 45.82 ± 0.73
(44-48) 		0 0 0 0 0 0 0 3 8 11 11 9
  60 45.89 ± 0.77
(44-48) 		0 0 0 0 0 0 0 5 11 16 16 14
  80 45.79 ± 0.86
(44-48) 		0 0 0 1 0 1 2 11 8 21 22 14
  100 46.03 ±0.76
(44-48) 		0 0 1 0 0 1 0 3 11 14 15 13
  0 45.83 ± 0.67
(44-48) 		0 0 0 0 0 0 0 10 9 19 19 14
  20 45.79 ± 0.64
(44-48) 		0 0 0 1 0 1 1 15 6 22 23 14
XPA-L 40 45.69 ± 0.99
(43-48) 		0 1 2 0 1 4 2 13 13 28 32 22
  60 45.65 ± 0.79
(43-48) 		3 0 1 1 0 5 2 12 14 28 33 22
  80 45.35 ± 1.46
(41-48) 		0 0 1 0 0 1 0 22 18 40 41 26
  100 45.77 ± 0.67
(44-48) 		0 0 3 1 0 4 5 16 15 36 40 28
  0 46.49 ± 0.97
(44-48) 		0 1 0 0 0 1 1 5 48 54 55 39
  20 46.28 ± 1.08
(44-48) 		0 1 1 0 0 2 0 5 44 49 51 37
XPB-L 40 46.61 ± 0.89
(44-48) 		0 1 0 0 0 1 0 6 34 40 41 29
  60 46.53 ± 0.87
(44-48) 		0 0 0 0 0 0 1 1 34 36 36 30
  80 46.43 ± 0.96
(44-48) 		0 1 4 0 1 7 1 8 51 60 67 40
  100 46.42 ± 1.01
(44-48) 		0 2 4 0 5 11 0 11 77 88 99 45
  0 45.86 ± 0.87
(44-48) 		0 0 0 0 0 0 3 21 16 40 40 30
  20 45.86 ± 0.87
(44-48) 		0 0 0 0 0 0 0 13 19 32 32 23
XPD-L 40 45.75 ± 0.86
(44-48) 		0 0 1 0 1 2 0 19 15 34 36 19
  60 45.95 ± 0.86
(44-48) 		2 1 1 0 0 4 6 19 31 56 60 37
  80 45.90 ± 0.91
(44-48) 		0 0 1 0 0 1 1 20 34 55 56 31
  100 45.80 ± 1.10
(44-48) 		0 1 1 1 0 3 3 10 17 30 33 25
  1. T/QLS-Triradial/Quadiradial-like structures; SCF-Sister Chromatid Fusions; D/T-Di/Tricentrics; R-Rings; EAF - End-to-end fusions of acentric fragments; CB-Chromsosome/Chromatid breaks; F-fragments including double minutes, terminal acentric fragments, interstitial acentric fragments and centric fragments; UT-Undetected telomeres. Data is represented as total number of aberrations. A total of 100 spreads were analyzed in two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, where significance is calculated to the respective cell type's untreated counterpart.
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Genome Integrity

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