Genome Integrity

Table 2 Analysis of metaphase spreads of lymphoblastoids following H2O2 treatment

From: Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

			Fusions						Breaks
Cell Type	H₂O₂(μM)	Chromosomes per metaphase	T/QLS	SCF	D/T	R	EAF	Total Fusions	CB	F	UT	Total Breaks	Total Aberrations	Aberrant cells (%)
	0	45.90 ± 0.68 (44-48)	0	0	0	0	0	0	0	1	6	7	7	7
	20	46.04 ± 0.49 (44-48)	0	1	0	0	1	2	0	1	10	11	13	10
Normal-L	40	45.82 ± 0.73 (44-48)	0	0	0	0	0	0	0	3	8	11	11	9
	60	45.89 ± 0.77 (44-48)	0	0	0	0	0	0	0	5	11	16	16	14
	80	45.79 ± 0.86 (44-48)	0	0	0	1	0	1	2	11	8	21	22	14
	100	46.03 ±0.76 (44-48)	0	0	1	0	0	1	0	3	11	14	15	13
	0	45.83 ± 0.67 (44-48)	0	0	0	0	0	0	0	10	9	19	19	14
	20	45.79 ± 0.64 (44-48)	0	0	0	1	0	1	1	15	6	22	23	14
XPA-L	40	45.69 ± 0.99 (43-48)	0	1	2	0	1	4	2	13	13	28	32	22
	60	45.65 ± 0.79 (43-48)	3	0	1	1	0	5	2	12	14	28	33	22
	80	45.35 ± 1.46 (41-48)	0	0	1	0	0	1	0	22	18	40	41	26
	100	45.77 ± 0.67 (44-48)	0	0	3	1	0	4	5	16	15	36	40	28
	0	46.49 ± 0.97 (44-48)	0	1	0	0	0	1	1	5	48	54	55	39
	20	46.28 ± 1.08 (44-48)	0	1	1	0	0	2	0	5	44	49	51	37
XPB-L	40	46.61 ± 0.89 (44-48)	0	1	0	0	0	1	0	6	34	40	41	29
	60	46.53 ± 0.87 (44-48)	0	0	0	0	0	0	1	1	34	36	36	30
	80	46.43 ± 0.96 (44-48)	0	1	4	0	1	7	1	8	51	60	67	40
	100	46.42 ± 1.01 (44-48)	0	2	4	0	5	11	0	11	77	88	99	45
	0	45.86 ± 0.87 (44-48)	0	0	0	0	0	0	3	21	16	40	40	30
	20	45.86 ± 0.87 (44-48)	0	0	0	0	0	0	0	13	19	32	32	23
XPD-L	40	45.75 ± 0.86 (44-48)	0	0	1	0	1	2	0	19	15	34	36	19
	60	45.95 ± 0.86 (44-48)	2	1	1	0	0	4	6	19	31	56	60	37
	80	45.90 ± 0.91 (44-48)	0	0	1	0	0	1	1	20	34	55	56	31
	100	45.80 ± 1.10 (44-48)	0	1	1	1	0	3	3	10	17	30	33	25

T/QLS-Triradial/Quadiradial-like structures; SCF-Sister Chromatid Fusions; D/T-Di/Tricentrics; R-Rings; EAF - End-to-end fusions of acentric fragments; CB-Chromsosome/Chromatid breaks; F-fragments including double minutes, terminal acentric fragments, interstitial acentric fragments and centric fragments; UT-Undetected telomeres. Data is represented as total number of aberrations. A total of 100 spreads were analyzed in two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, where significance is calculated to the respective cell type's untreated counterpart.

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