Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

Gopalakrishnan, Kalpana; Low, Grace Kah Mun; Ting, Aloysius Poh Leong; Srikanth, Prarthana; Slijepcevic, Predrag; Hande, M Prakash

doi:10.1186/2041-9414-1-16

Genome Integrity

Table 1 Cytokinesis-blocked micronucleus assay

From: Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

		Number of MN
Cells	H₂O₂(μM)	1	2	3	4	>4	% MN	% BN with MN
	0	2.80 ± 0.72	0.33 ± 0.12	0.13 ± 0.06	0.03 ± 0.06	0	4.00 ± 1.25	3.33 ± 0.86
	20	3.07 ± 0.60	0.47 ± 0.12	0	0.03 ± 0.06	0	4.13 ± 0.67	3.60 ± 0.62
Normal-L	40	3.63 ± 0.46	0.60 ± 0.27	0.13 ± 0.12	0.03 ± 0.06	0	5.37 ± 0.92	4.43 ± 0.64
	60	3.83 ± 1.21	0.53 ± 0.31	0.17 ± 0.06	0	0	5.40 ± 1.41	4.57 ± 1.30
	80	4.37 ± 0.74	0.57 ± 0.15	0.23 ± 0.06	0	0	6.20 ± 0.92	5.20 ± 0.85
	100	5.67 ± 0.46	0.87 ± 0.21	0.13 ± 0.06	0	0.03 ± 0.06	7.97 ± 0.96**	6.70 ± 0.70**
	0	2.33 ± 0.42	0.13 ± 0.15	0	0	0	2.60 ± 0.40	2.47 ± 0.38
	20	3.00 ± 0.35	0.30 ± 0.00	0	0	0	3.60 ± 0.35	3.30 ± 0.35
XPA-L	40	4.03 ± 1.20	0.80 ± 0.10	0.10 ± 0.10	0	0.07 ± 0.06	6.30 ± 1.35**	5.00 ± 1.20*
	60	4.00 ± 1.14	0.67 ± 0.25	0.10 ± 0.00	0.03 ± 0.06	0	5.77 ± 0.90*	4.80 ± 0.95
	80	5.43 ± 1.92	0.63 ± 0.21	0.17 ± 0.21	0	0	7.20 ± 2.04***	6.23 ± 1.89***
	100	5.90 ± 0.95	0.83 ± 0.12	0.23 ± 0.21	0.03 ± 0.06	0	8.40 ± 0.62***	7.00 ± 0.72***
	0	2.77 ± 0.95	0.43 ± 0.15	0	0	0	3.70 ± 1.20	3.20 ± 1.01
	20	3.10 ± 1.15	0.47 ± 0.21	0.10 ± 0.10	0	0	4.43 ± 1.65	3.67 ± 1.26
XPB-L	40	3.77 ± 0.85	0.37 ± 0.15	0.10 ± 0.10	0	0	4.87 ± 1.47	4.23 ± 1.08
	60	4.83 ± 0.55	0.43 ± 0.15	0.23 ± 0.12	0	0	6.53 ± 0.74*	5.50 ± 0.52
	80	5.57 ± 0.42	0.60 ± 0.10	0.23 ± 0.12	0	0	7.60 ± 0.35**	6.40 ± 0.44**
	100	6.00 ± 0.36	1.37 ± 0.15	0.37 ± 0.23	0	0	10.03 ± 1.00***	7.73 ± 0.46***
	0	5.10 ± 0.17	0.57 ± 0.12	0.03 ± 0.06	0	0	6.33 ± 0.58	5.70 ± 0.35
	20	7.07 ± 2.11	0.90 ± 0.17	0.17 ± 0.15	0.03 ± 0.06	0	9.50 ± 1.35*	8.17 ± 1.82
XPD-L	40	7.03 ± 2.14	0.90 ± 0.10	0.23 ± 0.21	0.03 ± 0.06	0	9.67 ± 2.44*	8.20 ± 2.25
	60	7.60 ± 0.62	0.90 ± 0.20	0.30 ± 0.17	0	0	10.30 ± 0.70**	8.80 ± 0.70**
	80	8.23 ± 1.56	1.20 ± 0.36	0.27 ± 0.21	0.03 ± 0.06	0	11.57 ± 0.76	9.73 ± 1.29***
	100	8.70 ± 2.19	1.17 ± 0.38	0.30 ± 0.10	0.07 ± 0.06	0.03 ± 0.00	12.47 ± 2.37***	10.27 ± 2.42***

Micronuclei (MN) scores, percentage of binucleated cells (BN) with MN and percentage of MN in cytokinesis-blocked binucleated lymphoblastoids following H₂O₂ treatment. Data is represented as mean ± SD. Statistics shown only for last two columns where *p < 0.05, **p < 0.01, ***p < 0.001, where significance is calculated to the respective cell type's untreated counterpart.

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