Skip to main content

Table 1 Cytokinesis-blocked micronucleus assay

From: Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

   Number of MN   
Cells H2O2(μM) 1 2 3 4 >4 % MN % BN with MN
  0 2.80 ± 0.72 0.33 ± 0.12 0.13 ± 0.06 0.03 ± 0.06 0 4.00 ± 1.25 3.33 ± 0.86
  20 3.07 ± 0.60 0.47 ± 0.12 0 0.03 ± 0.06 0 4.13 ± 0.67 3.60 ± 0.62
Normal-L 40 3.63 ± 0.46 0.60 ± 0.27 0.13 ± 0.12 0.03 ± 0.06 0 5.37 ± 0.92 4.43 ± 0.64
  60 3.83 ± 1.21 0.53 ± 0.31 0.17 ± 0.06 0 0 5.40 ± 1.41 4.57 ± 1.30
  80 4.37 ± 0.74 0.57 ± 0.15 0.23 ± 0.06 0 0 6.20 ± 0.92 5.20 ± 0.85
  100 5.67 ± 0.46 0.87 ± 0.21 0.13 ± 0.06 0 0.03 ± 0.06 7.97 ± 0.96** 6.70 ± 0.70**
  0 2.33 ± 0.42 0.13 ± 0.15 0 0 0 2.60 ± 0.40 2.47 ± 0.38
  20 3.00 ± 0.35 0.30 ± 0.00 0 0 0 3.60 ± 0.35 3.30 ± 0.35
XPA-L 40 4.03 ± 1.20 0.80 ± 0.10 0.10 ± 0.10 0 0.07 ± 0.06 6.30 ± 1.35** 5.00 ± 1.20*
  60 4.00 ± 1.14 0.67 ± 0.25 0.10 ± 0.00 0.03 ± 0.06 0 5.77 ± 0.90* 4.80 ± 0.95
  80 5.43 ± 1.92 0.63 ± 0.21 0.17 ± 0.21 0 0 7.20 ± 2.04*** 6.23 ± 1.89***
  100 5.90 ± 0.95 0.83 ± 0.12 0.23 ± 0.21 0.03 ± 0.06 0 8.40 ± 0.62*** 7.00 ± 0.72***
  0 2.77 ± 0.95 0.43 ± 0.15 0 0 0 3.70 ± 1.20 3.20 ± 1.01
  20 3.10 ± 1.15 0.47 ± 0.21 0.10 ± 0.10 0 0 4.43 ± 1.65 3.67 ± 1.26
XPB-L 40 3.77 ± 0.85 0.37 ± 0.15 0.10 ± 0.10 0 0 4.87 ± 1.47 4.23 ± 1.08
  60 4.83 ± 0.55 0.43 ± 0.15 0.23 ± 0.12 0 0 6.53 ± 0.74* 5.50 ± 0.52
  80 5.57 ± 0.42 0.60 ± 0.10 0.23 ± 0.12 0 0 7.60 ± 0.35** 6.40 ± 0.44**
  100 6.00 ± 0.36 1.37 ± 0.15 0.37 ± 0.23 0 0 10.03 ± 1.00*** 7.73 ± 0.46***
  0 5.10 ± 0.17 0.57 ± 0.12 0.03 ± 0.06 0 0 6.33 ± 0.58 5.70 ± 0.35
  20 7.07 ± 2.11 0.90 ± 0.17 0.17 ± 0.15 0.03 ± 0.06 0 9.50 ± 1.35* 8.17 ± 1.82
XPD-L 40 7.03 ± 2.14 0.90 ± 0.10 0.23 ± 0.21 0.03 ± 0.06 0 9.67 ± 2.44* 8.20 ± 2.25
  60 7.60 ± 0.62 0.90 ± 0.20 0.30 ± 0.17 0 0 10.30 ± 0.70** 8.80 ± 0.70**
  80 8.23 ± 1.56 1.20 ± 0.36 0.27 ± 0.21 0.03 ± 0.06 0 11.57 ± 0.76 9.73 ± 1.29***
  100 8.70 ± 2.19 1.17 ± 0.38 0.30 ± 0.10 0.07 ± 0.06 0.03 ± 0.00 12.47 ± 2.37*** 10.27 ± 2.42***
  1. Micronuclei (MN) scores, percentage of binucleated cells (BN) with MN and percentage of MN in cytokinesis-blocked binucleated lymphoblastoids following H2O2 treatment. Data is represented as mean ± SD. Statistics shown only for last two columns where *p < 0.05, **p < 0.01, ***p < 0.001, where significance is calculated to the respective cell type's untreated counterpart.