Figure 2

Experimental Design. A schematic diagram of a simple nutrient array design that was used to study the interactive effects of folic acid (F) and riboflavin (R) at low (L) and high (H) physiological concentration. In this study by Kimura et al 2004 [28] the folic acid-riboflavin interactive effects in four different combinations (LFLR, LFHR, HFLR, HFHR) on DNA damage were measured using the CBMNcyt assay in lymphocytes that were homozygous for the common or rarer allele of the C677T polymorphism in the methylenetetrahydrofolatereductase (MTHFR) gene. Riboflavin is a precursor of the FAD cofactor for MTHFR and folic acid is a precursor for 5,10-methylenetetrahydrofolate the substrate for MTHFR. Cell growth and homocysteine were also measured. N = 7 C677C, N = 7 T677T