|
Treatment
|
No. metaphases analyzed
|
No. metaphases with aberrations
|
No. of chromatid breaks
|
|---|
|
Control (+DMSO)
| | | |
|
0 Gy
|
55
|
1
|
1
|
|
0.02 Gy
|
59
|
7
|
8
|
|
0.04 Gy
|
61
|
3
|
3
|
|
0.08 Gy
|
75
|
11
|
16
|
|
0.1 Gy
|
70
|
15
|
21
|
|
0.2 Gy
|
50
|
14
|
18
|
|
0.4 Gy
|
55
|
25
|
53
|
|
+KU55933
| | | |
|
0 Gy
|
54
|
0
|
0
|
|
0.02 Gy
|
56
|
9
|
9
|
|
0.04 Gy
|
64
|
5
|
5
|
|
0.08 Gy
|
61
|
11
|
15
|
|
0.1 Gy
|
53
|
19
|
25
|
|
0.2 Gy
|
50
|
31
|
42
|
|
0.4 Gy
|
51
|
42
|
106
|
- Cells were treated with KU55933 (10 μM) 30 minutes before irradiation until 30 minutes before metaphase harvest. Immediately after irradiation, colcemid (0.1 μM) was added to the medium, and cells were incubated for 2 hours. Then, metaphases were collected and chromosome samples were prepared as described in Methods.
