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Table 1 chromatid breaks induced by various doses of x-rays

From: Image-based quantitative determination of DNA damage signal reveals a threshold for G2 checkpoint activation in response to ionizing radiation

Treatment No. metaphases analyzed No. metaphases with aberrations No. of chromatid breaks
Control (+DMSO)    
0 Gy 55 1 1
0.02 Gy 59 7 8
0.04 Gy 61 3 3
0.08 Gy 75 11 16
0.1 Gy 70 15 21
0.2 Gy 50 14 18
0.4 Gy 55 25 53
+KU55933    
0 Gy 54 0 0
0.02 Gy 56 9 9
0.04 Gy 64 5 5
0.08 Gy 61 11 15
0.1 Gy 53 19 25
0.2 Gy 50 31 42
0.4 Gy 51 42 106
  1. Cells were treated with KU55933 (10 μM) 30 minutes before irradiation until 30 minutes before metaphase harvest. Immediately after irradiation, colcemid (0.1 μM) was added to the medium, and cells were incubated for 2 hours. Then, metaphases were collected and chromosome samples were prepared as described in Methods.